Facilities

Flow cytometry service

Applications


  • Multi-parameter analysis of both extra and intracellular labelling for cellular immunophenotyping and functionality.


  • Gene expression analysis using reporter genes.


  • Measure of cell proliferation: CFDA, BrdU, CFSE, etc.


  • Cell cycle: Cyclins, DNA content


  • Apoptosis assays (plasma membrane integrity, mitochondrial function, caspase activity, DNA fragmentation, etc.)


  • Cytokine secretion by multiplex assay


  • FRET.


  • Separation of cell subpopulations based on the differential expression of various intra and extracellular markers.


Ongoing Applications


  • Protein localization using PulSA methodology.


  • Development of new cell separation applications by cell sorting.


  • Separation of large particles, as yeast clusters or small larvae of C. Elegans.


  • Separation of small particles, below 1um based on their phenotypic characteristics eg microvesicles.


  • High dimensional data analysis , such as dimensionality reduction and clustering algorithms using Cytobank.


Tecnical Support


  • New User Equipment Management Course.


  • General monitoring and maintenance of analysers.


  • Help users with equipment problems, whether technical or experimental.


  • Support on experimental design and methodology.
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